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Jun 01, · The cells were cultured at 37°C under a humidified atmosphere of 5% CO 2-95% air in 75-cm 2 tissue culture flasks until cells reached preconfluence. Cell purity was confirmed by the appearance of typical cobblestone morphology and the presence of von Willebrand factor antigen. Cells were used between passages 1 and 3.

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Optimum function of HLA-DR molecules in transgenic mice requires efficient interaction between the class II molecules on APCs and CD4 on T cells. Residues 110 and 139 of the second domain of class II molecules are considered to be critical for recognition of CD4. We generated an HLA-DR4beta(NT) tran GP11F Cone Crusher Parts

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